Table II.
Thermocycling conditions used for PCR.
| Stage | Cycles | Temperature, °C | Duration |
|---|---|---|---|
| Initial denaturation | 1 | 95 | 5 min |
| Thermocycling | 40 | 95 | 10 sec |
| 60 | 30 sec | ||
| Solubility curve | 1 | 95 | 15 sec |
| 60 | 60 sec | ||
| 95 | 15 sec |
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