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. 2021 Aug 2;9:687524. doi: 10.3389/fcell.2021.687524

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Copyright © 2021 Chen, Sun, Dai, Li, Yan, Zhang, Xiao, Shen, Huang, Xiang, Zhang, Tan, Yang, Nie, Huang, Lian and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRNDE enhances the stability of ATG4B mRNA. (A) Subcellular distribution of CRNDE in HCC cells was assayed by FISH (Scale bar: 10 μm). (B) The levels of nuclear and cytoplasmic CRNDE in HCC cells were tested by qPCR. GAPDH RNA and U6 RNA were used as cytoplasmic and nuclear RNA controls, respectively. (C) The level of CRNDE in the co-immunoprecipitates with anti-ATG4B antibody in HepG2 cells was examined by RIP assay. SNRNP70 (binding to U1 snRNA) and IgG were used as positive control and negative control, respectively. (D,E) After transfected with pcDNA-CRNDE (or pcDNA3.1) (D) or si-CRNDE (or si-NC) (E) for 18 h, HepG2 and Hep3B cells were treated with 5 μg/mL actinomycin D (Act D) for the indicated times, and then the level of ATG4B mRNA was detected by qPCR. pcDNA-CRNDE, pcDNA3.1, si-CRNDE and si-NC were the same as the description in Figure 1; ns, no significance; *P < 0.05.