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Respiration Buffer (B)
| Reagent | Final concentration | Amount |
|---|---|---|
| Sucrose | 225 mM | 7.7 g |
| KCl | 10 mM | 74 mg |
| MgCl2 | 5 mM | 47.6 mg |
| HK2PO4 | 10 mM | 134 mg |
| H2KPO4 | 10 mM | 30 mg |
| EGTA | 1 mM | 38 mg |
| Tris HCl | 10 mM | 121 mg |
| BSA | 0.05% | 50 mg |
| H2O milli-Q | n/a | up to 100 mL |
| Total | n/a | 100 mL |
Storage at −20°C until use. Use at 30°C.
