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. 2021 Jul 19;64(15):11496–11526. doi: 10.1021/acs.jmedchem.1c00806

Figure 4.

Figure 4

Compound 27a moderately inhibits mPGES-1 within eicosanoid and docosanoid biosyntheses. (A–G) Effect of 27a on mPGES-1 in microsomal preparations of IL-1β-treated A549 cells (A), 12-LOX (B), and 15-LOX product formation (C) in PMNL treated with A23187 and AA, isolated ovine COX-1 (D), COX-1-dependent 12-HHT formation in platelets (E), human recombinant COX-2 (F), and human recombinant sEH (G). (H) Quantitative illustration of the lipid mediator network of macrophages (M1) treated with 27a (1 μM) and stimulated with pathogenic E. coli as compared to the vehicle control. The node size represents the mean concentration in pg, and the color intensity denotes the fold change for each lipid mediator. AA, arachidonic acid; DHA, docosahexaenoic acid; cys-LTs, cysteinyl-LTs; et-LTB4, LTB4 isomers; EPA, eicosapentaenoic acid; LX, lipoxine; MaR, maresin; 20-OH-LTB4, 20-hydroxy-LTB4; PD, protectin; Rv, resolvin; TX, thromboxane. Data are expressed as mean (H) + SEM (A-G) from n = 3 independent experiments. *p < 0.05 vs control; RM one-way ANOVA + Tukey’s post hoc test.