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. 2021 Jul 19;64(15):11496–11526. doi: 10.1021/acs.jmedchem.1c00806

Figure 6.

Figure 6

Compound 27a relieves inflammation in reconstructed human epidermis (RHE). RHE exposed to 27a or dexamethasone (dex) was treated with a cytokine cocktail for 4 days to trigger the inflammatory reaction. (A) Concentration of IL-8 in the growth medium. The dotted line indicates basal levels without cytokine stress. (B) Morphological changes visualized by hematoxylin and eosin staining (scale bar is 50 μm; representative of three independent experiments). (C) Transepidermal water loss (TEWL) at the RHE surface. (D) Impermeability of the stratum corneum. The stratum corneum of cytokine-stressed RHE becomes permeable for Lucifer yellow (green) that diffuses into the viable cell layers, as shown in the insets in higher magnification (scale bar for the outer box is 20 μm, and scale bar for the inset is 10 μm; representative of three independent experiments). Nuclei were stained with 4′,6′-diamidino-2-phenylindole (DAPI, blue). (E) pH value at the RHE surface. Data are expressed as mean ± SEM (A, 27a) with single values (A, dex, C) or mean + floating bars from minimum to maximum values (E) from n = 2 (A) and n = 3 (C, E) independent experiments. Ordinary one-way ANOVA + Tukey’s post hoc test.