Figure 4.

The Dek47 mutant is deficient for mitochondrial nad2 mature transcript. (A) RT-PCR analysis of transcript levels of 35 mitochondrion-encoded genes in WT and dek47-1. RNA was isolated from the same ear segregating for WT and dek47-1. Normalization was performed against ZmActin. (B) Quantitative RT-PCR analysis of 35 mitochondrion-encoded transcripts in WT and dek47-1. RNA samples were normalized against ZmActin. Values represent the mean and standard deviation of three biological replicates, ±SD. The comparison groups of the Student’s t-test are wild type and dek47 mutant. Asterisks indicate significant differences between means calculated with Student’s t-test. ^*p < 0.05; ^**p < 0.01.