Figure 6.

DEK47 can rescue the Arabidopsis rug3 mutant phenotypes. (A) Phenotype comparison of Col-0, rug3, Dek47/rug3-1 (Com1), and Dek47/rug3-2 (Com2). (B) Quantitative RT-PCR analysis of intron splicing efficiency of nad2 transcript in WT, rug3, Com1, and Com2. RNA samples were normalized against Actin gene ACT2 (AT3G18780). Values represent the mean and standard deviation of three biological replicates, ±SD. The comparison groups of the Student’s t-test are between rug3 mutant and wild type, and between complemented line and wild type, respectively. Asterisks indicate significant differences between means calculated with Student’s t-test. ^*p < 0.05; ^**p < 0.01.