FIGURE 1.

Effect of melatonin on HT22 cell viability after OGD/R treatment. A, the percentage of viable cells analyzed by the Trypan Blue exclusion test in untreated cells (Ctrl), 8 h OGD‐exposed cells followed by 18 h reoxygenation (OGD/R) and 8 h OGD‐exposed cells followed by 18 h reoxygenation in the presence of 50 µmol/L melatonin (OGD/R+Mel). B, 5‐CFDA mean fluorescence intensity (MFI) in Ctrl, OGD/R, and OGD/R+Mel cells. Mean values were converted to arbitrary units (AU) setting control as 100. C, morphological changes in Ctrl, OGD/R, and OGD/R+Mel cells acquired by an optical microscope with 10× objective. Each value is expressed as percentage ±SD (N = 3 independent experiments performed in triplicate); *P < .05, ***P < .001 vs Ctrl; # P < .05 vs OGD/R+Mel