FIGURE 3.

Activation of WNT, SHH, and EDA signaling improved HF-related marker expression in KC-DP constructs. (A) Addition of the activators of signaling pathways involved in HF morphogenesis did not evidently ameliorate immunohistochemically detected HF marker expression in KC-DP constructs. *DP aggregates. (B) HF-related gene expression levels analyzed by qRT-PCR in KC-DP constructs tended to be increased by a WNT agonist (WNT) treatment, which tended to be further enhanced by the treatment by the mixture of agonists (WNT + SHH + EDA) (Mean ± SEM; n = 3, triplicate for each experimentation; Wilcoxon signed-rank test). KC, keratinocyte; DP, dermal papilla, Scale bar: 200 μm.