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. 2021 Aug 2;9:590333. doi: 10.3389/fcell.2021.590333

FIGURE 5.

FIGURE 5

KC-iDP constructs reproduced architectural/biochemical characteristics and gene expression profile of KC-DP constructs. (A) Morphology of KC-iDP constructs. Scale bar: 200 μm. (B) The schema illustrating the intratissue structure of KC-iDP constructs. (C) Histological characteristics of KC-iDP constructs resembling those of KC-DP constructs (see Figure 2). *: iDP aggregates. Scale bar: 200 μm. (D) Immunohistochemical analysis revealed that HF keratin distribution was almost indistinguishable between KC-DP and KC-iDP constructs (see Figure 2). KRT40-positive cells rearranged in the center of the main body mimicking the hair shaft without being splitted by a nylon fiber (the rightmost panel). *: iDP aggregates. Scale bar: 200 μm. (E) Immunoreactivities for mesenchymal markers in KC-iDP constructs. *: iDP aggregates. Scale bar: 200 μm; DP, dermal papilla; KC, keratinocyte; KRT, keratin; HF, hair follicle; iDP, human induced pluripotent cell-derived dermal papilla substituting cell aggregate; IRS, inner root sheath; ORS, outer root sheath; SMA, smooth muscle actin; VCAN, versican; VIM, vimentin.