Table 3.
Primer sequences for RT-qPCR
| Targets | Sequences (5′-3′) |
|---|---|
| miR-214 | F: AGAGTTGTCATGTGTCT |
| R: GAACATGTCTGCGTATCTC | |
| ROCK1 | F: CACGCCTAACTGACAAGCACCA |
| R: CAGGTCAACATCTAGCATGGAAC | |
| SOX4 | F: GATCTCCAAGCGGCTAGGCAAA |
| R: GATCTCCAAGCGGCTAGGCAAA | |
| SEMA4C | F: GGAGTATGACTGCTATTCCGAGC |
| R: ACACCAACCGAGCCTTCAGGAA | |
| PPTC7 | F: GCGGTTAGTGAAAGAAGGACGC |
| R: TTCTGTCCAGCACCACGATGCA | |
| U6 | F: CTCGCTTCGGCAGCACAT |
| R: TTTGCGTGTCATCCTTGCG | |
| GAPDH | F: CATCACTGCCACCCAGAAGACTG |
| R: ATGCCAGTGAGCTTCCCGTTCAG |
Notes: RT-qPCR Reverse transcription quantitative polymerase chain reaction, F Forward, R Reverse, miR-214 microRNA-214, ROCK1 Rho-associated kinase 1, SOX4 SRY-box transcription factor 4, SEMA4C Semaphorin 4C, PPTC7 Protein phosphatase targeting COQ7, GAPDH Glyceraldehyde-3-phosphate dehydrogenase