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Table 2.
Mobile phase condition of HPLC
| Times (min) | Methanol (A) | 0.1% Phosphoric acid |
|---|---|---|
| 0 | 8 | 92 |
| 120 | 65 | 35 |
| 121 | 8 | 92 |
| 135 | 8 | 92 |