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In vitro anti-tumor effects of different drug formulations in 4T1 cells. IC50 was calculated to evaluate the cytotoxicity of various drug formulations after incubation with 4T1 cells for 24 h under a normoxia and b hypoxia. c Apoptosis of 4T1 cells treated with different drug formulations for 24 h was detected by Annexin V-FITC/PI double staining. Cells treated with PBS were used as control. Data are expressed as mean ± SD (n = 3), *p < 0.05, ***p < 0.001
