Table 1. Seventeen Fragments Identified in the First “Triage” Round of Screening, in a Binary Yes/No Fashion, from the Compound Mixture Library^a.

^aFollow up screening steps provided a qualitative ranking of the fragments, based on the fractional bound occupancy (1 – I_B/I_F). Compounds that show fractional bound occupancy greater than 0.4 are shown to bind to the RNA in NOESY experiments; therefore, a general cutoff of 0.4 is applied to separate and positively confirm RNA binding fragments.