Table 2.

Comparison of slop, amplification efficiency, and R² estimated through the linear regression of triple standard curves of Cel-miRNA-39-1 according to the annealing temperature

Parameters	Annealing temp. (℃)
	58	60	62
Slope
Median	− 4.144	− 3.554	− 3.648
Range	− 4.5042 to − 3.8800	− 3.5939 to − 3.3490	− 4.1735 to − 3.6066
95% CI	− 4.953 to − 3.396	− 3.825 to − 3.173	− 4.594 to − 3.024
Efficiency (%)
Median	74.40	91.15	62.01
Range	66.7 to 81.0	89.8 to 98.9	73.6 to 89.3
95% CI	56.29 to 91.81	81.08 to 105.5	62.01 to 105.3
R2
Median	0.9970	0.9993	0.9962
Range	0.9820 to 0.9993	0.9907 to 0.9999	0.9899 to 0.9973
95% CI	0.9696 to 1.016	0.9838 to 1.009	0.9845 to 1.004