Table 3.

Effect of multiple chloroform extraction and ethanol washing on elimination of RNA yield and phenol (n = 3)

Extraction and washing condition	RNA concentration(ng/µL) Median (range)	Detected phenol contaminant at 260 nm Median (range)
Once chloroform extraction without EtOH washing	294.0 (240.7–381.45)	6.44 (5.15–8.23)
Once chloroform extraction and EtOH washing	152.4 (150.0–175.9)	3.43 (2.13–4.00)
Ttriple chloroform extraction without EtOH washing	201.3 (158.0–215.0)	4.00 (2.94–4.08)
Triple chloroform extraction and EtOH washing	130.2 (102.8–158.1)	2.63 (2.07–2.95)