FIGURE 4.

PDOTS preserve diverse stromal elements. (A) CD3+ T‐cell preservation in Passage 0 (P0) PDOTS culture day 5 and in Passage 2 (P2) PDOTS culture day 4. Green, EpCAM+ epithelial cells; Red, CD3+ T cells; Blue, DAPI. (B) Various levels of stromal elements in Passage 0 (P0) PDOTS culture day 5. First panel figures were adapted from (A) upper panel. Red, EpCAM+ epithelial cells; Green, CD45+ T cells; Green, CD8+ T cells, Green, CD68+ macrophages, and Green, CD31+ endothelial cells; Blue, DAPI. Scale bar, 100 μm