Figure 2.

Regulatory T cells (Tregs) are involved in protective tolerance induced by MOG-peptide 35–55 (pMOG)-polyethylene glycol (PEG)20. Fourteen days prior to experimental autoimmune encephalomyelitis (EAE) induction, C57BL/6 mice were tolerized with phosphate buffered saline (PBS) (control), 7.6 µg pMOG or equimolar amounts of pMOG-PEG20. After 7 days mice received either 500 µg anti-CD25 (PC61) antibody or PBS (control) intraperitoneally (i.p.). EAE was induced as described above. Mean clinical score per group ± standard error of the mean (SEM), n = 8 animals per group except pMOG-PEG/anti CD25 (n = 7; one animal had to be removed for ethical reasons (ascites after pertussis toxin)). One representative of 4 independent experiments (the others being depicted in Supplemental Figure 4) is shown. For statistics see Supplemental Table 1 in the Supplemental material.