Figure 4.

Reduced central nervous system (CNS) infiltration at peak of disease following MOG-peptide 35–55 (pMOG)-polyethylene glycol (PEG)20 vaccination. Seven days prior to experimental autoimmune encephalomyelitis (EAE) induction mice were tolerized with phosphate buffered saline (PBS), 7.6 µg pMOG or equimolar amounts of pMOG-PEG20. At the peak of disease (day 15) spinal cords were isolated from pMOG-immunized C57BL/6 mice as well as from non-immunized untreated C57BL/6 mice (control). Different leukocyte subsets were quantified by flow cytometric analysis of marker combinations as described in the Methods and Supplemental Figure 6. Data are depicted as mean ± standard deviation (SD) (n = 6–12). Data are summarized from two independent experiments. For frequencies of cell populations and statistical data see Supplemental material, Supplemental Figure 7 and Supplemental Table 2.