Extended Data Figure 4 |. Cryo-EM Analysis of S2-connected duplex pairs from non-homologous dsDNA reactions.

a, The 5’ end of the filament, but not the 3’ end produces classes with conspicuously short duplexes. Reconstruction after 3D refinement of paired duplexes that have duplex^H or duplex^I. Top, maps of classes with short duplexes. Bottom, maps of classes from the same 3D classification but with regular, long duplexes. Black circle highlights the difference in the duplex length. Maps are colored by the duplex tilt (5’ cyan, 3’ purple), S2 DNA red. Overall resolution, from gold-standard refinement procedure, and particle numbers are also shown.

b, Charts show the number of particles with S2-connected duplex pairs for the non-homologous 67 bp dsDNA dataset. Each chart is of the series that contains the 1^st duplex in common. Pairs of duplexes starting at RecA^C are omitted, as they are shown in Fig 1d. The data set was collected once.

c, Charts show the number of particles with S2-connected duplex pairs for the non-homologous 120 bp dsDNA dataset as in b. Pairs of duplexes starting at RecA^C are omitted as they are shown in Fig 1l. The data set was collected once.