Figure 1.

Differentiation of human Th22 cells in vitro. (A) IL-22 and IL-17 in the supernatant of naïve CD4⁺ T cells co-cultured with allogeneic DC for 96 h in the absence or presence of TNFα (10 ng/ml), IL-1β (10 ng/ml), IL-6 (30 ng/ml) IL-23 (20 ng/ml), FICZ (0.3 µM) and TGFβR inhibitor (galunisertib,10 µM), (mean + SEM, two independent experiments with 5 donors). (B) IL-22 in the supernatant of DC-T cell co-cultures, T cell mono-cultures activated with CD3/CD28 beads and DC mono-cultures after 24-144 h of culture in Th22 medium (mean ± SEM, two independent experiments with 4 donors).