Figure 3.

1,25(OH)₂D₃ inhibits IL-22 production in human Th22 cells. (A) Relative mRNA expression (B) and production of IL-22 in naïve CD4⁺ T cells co-cultured with allogeneic DC for 96 h in Th22 medium and the indicated concentrations of 1,25(OH)₂D₃. The data in (A) are normalized to the values obtained from DC-TC co-cultures incubated in Th22 medium in the absence of 1,25(OH)₂D₃ (mean + SEM, six independent experiments with 10 donors). (C) Frequency of IL-22⁺ CD25⁺CD4⁺ T cells after activation of naïve CD4⁺ T cells with allogeneic DC for 96 h in Th22 medium and the indicated concentrations of 1,25(OH)₂D₃ (mean percentage of positive cells + SEM, three independent experiments with 5 donors). (D) Mean fluorescent intensity (MFI) of IL-22 in the IL22⁺CD25⁺ T cells described above (mean expression of IL-22 + SEM, three independent experiments with 5 donors). (E) Relative mRNA expression and (F) IL-22 production in naïve CD4⁺ T cells from healthy individuals (black) and from the HVDRR patient (white) activated with allogeneic DC for 96 h in Th22 medium and the indicated concentrations of 1,25(OH)₂D₃. The data are normalized to the values obtained from CD4⁺ T cells activated in the absence of 1,25(OH)₂D₃ (mean + SEM, two independent experiments with 4 donors).