Figure 4.

1,25(OH)₂D₃ does not inhibit IL-22 by affecting AhR, RORγt or STAT-3 expression. (A) Relative AhR mRNA expression and (B) representative Western Blot (lower panel) and quantification (upper panel) of AhR with GAPDH as loading control from naïve CD4⁺ T cells co-cultured with allogeneic DC for 96 h in Th22 medium and the indicated concentrations of 1,25(OH)₂D₃. (C) Relative RORC mRNA expression and (D) representative Western Blot (lower panel) and quantification (upper panel) of RORγt with GAPDH as loading control from naïve CD4⁺ T cells co-cultured with allogeneic DC for 96 h in Th22 medium and the indicated concentrations of 1,25(OH)₂D₃. (E) Relative STAT-3 mRNA expression and (F) representative Western Blot (lower panel) and quantification (upper panel) of STAT-3 and phosphorylated STAT-3 (p-STAT3) with GAPDH as loading control from naïve CD4⁺ T cells co-cultured with allogeneic DC for 96 h in Th22 medium and the indicated concentrations of 1,25(OH)₂D₃. The data are normalized to the values obtained from DC-T cell co-cultures incubated in Th22 medium in the absence of 1,25(OH)₂D₃. (mean + SEM, two experiment with 4 donors).