Table 1. All used Primers, including Illumina sequencing adapters and developed Tuna blocking primer of this study, produced by Eurofins (Ebersberg, Germany).
| Name | Direction | Region | Amplicon size bp | Primer Sequence 5′–3′ | Publication |
|---|---|---|---|---|---|
| mlCOIintF | Forward | Mini-COI | 313 | GGWACWGGWTGAACWGTWTAYCCYCC | Leray et al. (2013b) |
| jgHCO2198 | Reverse | Mini-COI | TANACYTCNGGRTGNCCRAARAAYCA | Geller, Meyer & Parker (2013) | |
| SSUF04 | Forward | 18S V1-V2 | 356 | GCTTGTCTCAAAGATTAAGCC | Blaxter et al. (1998) |
| SSURmod | Reverse | 18S V1-V2 | CCTGCTGCCTTCCTTRGA | Sinniger et al. (2016) | |
| Adapter | Forward | – | – | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGMK | ownership Illumina® |
| Adapter | Reverse | – | – | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGMK | ownership Illumina® |
| ThunB1 | Forward | Mini-COI | Blocking | AACCGGTTGAACAGTCTACCCTCCCCTTGCCGGC-SpC3I | this study |