Table 1.
RT-qPCR primers for intestinal target bacteria.
| Target bacteria | Target gene | Amplicon length (bp) | Primer sequences (5′-3′) | Tm (°C) |
|---|---|---|---|---|
| (F, forward; R, reverse) | ||||
| Clostridium (Da Silva et al. 2018) | 16S rRNA | 86 | F: CATCCTGATGACGGTTTCTTAACC | 58 |
| R: GTTGCGGGACTTAACCCA | ||||
| Bacteroides (Jiang et al. 2014) | 16S rRNA | 202 | F: CTGAACCAGCCAAGTAGCG | 58 |
| R: CCGCAAACTTTCACAACTGACTTA | ||||
| Sutterella (Williams et al. 2012) | 16S rRNA | 234 | F: CGCGAAAAACCTTACCTAGCC | 62 |
| R: GACGTGTGAGGCCCTAGCC | ||||
| Bacteroides uniformis (Tong et al. 2011) | 16S rRNA | 112 | F: TCTTCCGCATGGTAGAACTATTA | 60 |
| R: ACCGTGTCTCAGTTCCAATGTG | ||||
| Mucispirillum schaedleri | 16S rRNA | 178 | F: CGAGCGTTGTTCGGAGTGACTG | 65 |
| R: CCAGCCAGATTGCCGCCTTC | ||||
| Faecalibacterium prausnitzii (Bartosch et al. 2004) | 16S rRNA | 177 | F: GATGGCCTCGCGTCCGATTAG | 60 |
| R: CCGAAGACCTTCTTCCTCC | ||||
| Ruminococcus gnavus (Cao et al. 2016) | 16S rRNA | 103 | F: GGACTGCATCGTCCAGAAAG | 58 |
| R: AACGTCAGTCATCGTCCAGAAAG |