Video 1.
Tomogram and model of a cell pair prefusion. The video shows all virtual z-sections of the tomographic reconstruction of a prefusion site at the close CW contact stage. The PM is segmented in cyan, vesicles are shown in pink (large vesicles) and purple (small vesicles), and filaments are segmented with a yellow line. The left cell shows a convex shape with a sPM. The cell on the right shows a slightly concave shape at the site of cell–cell contact with PM that exhibits an undulating pattern. Scale bar, 100 nm.