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. 2021 Aug 12;15:3509–3522. doi: 10.2147/DDDT.S315249

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© 2021 Wei et al.

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Quercetin alleviates cellular senescence in vitro. (A) mRNA expression levels of senescence markers (p21 and p53) in hPDLCs treated with 200 μM H₂O₂ for 1 h, 6 h and 12 h. (B) Representative SA-β-gal staining images and quantification of cells in the different treatment groups. Scale bar: 50 μm. (C) mRNA expression levels of senescence markers (p21 and p53) in hPDLCs after quercetin treatment. (D) Representative Western blotting images of p21 protein and quantification in hPDLCs. (E) Representative images and quantification of γH2AX-positive cells stained with DAPI after different treatments (green: γH2AX; blue: DAPI). Scale bar: 50 μm. All data are presented as the mean ± SD, *p < 0.05.