Table 2. The primer sequences and positive controls of pathogens.

Pathogens	Target gene	Size	Sequence (5′ to 3′)	Positive control	References for reaction materials and PCR condition
Bacillus cereus	Bal	533	BalF: 5′-TGCAACTGTATTAGCACAAGCT-3′	B. cereus, ATCC 11778	[41]
			BalR: 5′-TACCACGAAGTTTGTTCACTACT-3′
Clostridium difficile	tcdB	632	tcdA-F 5′-GTATGGATAGGTGGAGAAGTCAGTG-3′	C. difficile, ATCC 9689	[42]
			tcdA-R 5′-CGGTCTAGTCCAATAGAGCTAGGTC-3′
	tcdA	441	tcdB-F 5′-GAAGATTTAGGAAATGAAGAAGGTGA-3′
			tcdB-R 5′-AACCACTATATTCAACTGCTTGTCC-3′
	cdtA	260	cdtA-F 5′-ATGCACAAGACTTACAAAGCTATAGTG-3′
			cdtA-R 5′-CGAGAATTTGCTTCTATTTGATAATC-3′
	cdtB	179	cdtB-F 5′-ATTGGCAATAATCTATCTCCTGGA-3′
			cdtB-R 5′-CCAAAATTTCCACTTACTTGTGTTG-3′
Vibrio cholera	ompW	427	VCompW-F: 5′-CACCAAGAAGGTGACTTTATTGTG-3′	V. cholera, ATCC 14035	[43]
			VCompW-R: 5′-CGTTAGCAGCAAGTCCCCAT-3′
V. parahaemolyticus	Collagenase	271	VPC-F: 5′-GAAAGTTGAACATCATCAGCACGA-3′	V. parahaemolyticus, ATCC 17802	[44]
			VPC-R: 5′-GGTCAGAATCAAACGCCG-3′
V. vulnificus	vvhA	505	FDAvvhA-F: 5′ -CCGCGGTACAGGTTGGCGCA-3′	V. vulnificus, ATCC 27562	[43]
			FDAvvhA-R: 5′-CGCCACCCACTTTCGGGCC-3′
Hepatitis A virus	3D	412	HAV1: 5′-TTTGGTTGGATGAAAATGGTT-3′	Internal controls:	[45]
			HAV4: 5′-ATTCTACCTGCTTCTCTAATC-3′
		233	HAV2: 5′-CAACCTGTCCAAAAGATGAAT-3′
			HAV3: 5′-ACCAACATCTCCGAATCTTA-3′
Norovirus	ORF2		Group I, GI:		[46]
			COG1F: 5′-CGYTGGATGCGNTTYCATGA-3′
		377	G1-SKF: 5′-CTGCCCGAATTYGTAAATGA-3′
		330	G1-SKR: 5′-CCAACCCARCCATTRTACA-3′
		386	Group II GII:	Bacteria universal 16S rDNA PCR for avoiding false negative results
		344	COG2F: 5′- CARGARBCNATGTTYAGRTGGATGAG-3′
			G2-SKF: 5′- CNTGGGAGGGCGATCGCAA -3′
			G2-SKR: 5′- CCRCCNGCATRHCCRTTRTACAT -3′
Adenovirus	Hexon neHexon	301	Hex:		[47]
			Hex1 deg F: 5′-GCCSCARTGGKCWTACATGCACATC-3′
			Hex2 deg R:5′-CAGCACSCCICGRATGTCAAA-3′
		171	neHex:
			neHex 3deg F: 5′-GCCCGYGCMACIGAIACSTACTTC-3′
			neHex 4deg R: 5′-CCYACRGCCAGIGTRWAICGMRCYTTGTA-3′