KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Bacterial and virus strains
Biological samples
Chemicals, peptides, and recombinant proteins
Schneider’s Drosophila Media	Thermo Fisher	Cat#21720024
Clostripain	Alfa Aesar, Thermo Fisher	Cat#J61362
Trypsin	Gibco, Life Technologies	Cat# 25300054
Gbp1–5	This paper	https://www.genscript.com/peptide-services.html?
Bbsl	New England Biolabs	Cat#R3539S
Critical commercial assays
Deposited data
Experimental models: cell lines
Experimental models: organisms/strains
See Table S1	Various	N.A.
See Table S2	Various	N.A.
Oligonucleotides
Gbp1Δ gRNA#1: ATTTGCTCCCATCATTTATC	Millipore Sigma	https://www.sigmaaldrich.com/
Gbp1Δ gRNA#2: CGGAAAACGATGCAGAAAGC	Millipore Sigma	https://www.sigmaaldrich.com/
Gbp23ΔFS gRNA#1: GAATATTCAACGCTGCCGTT	Millipore Sigma	https://www.sigmaaldrich.com/
Gbp23ΔFS gRNA#2: AATTCCATACAACCGCGTCC	Millipore Sigma	https://www.sigmaaldrich.com/
Recombinant DNA
pGP-CMV-GCaMP6m	Chen, et al., 2013	Addgene Plasmid #40754
Actin5c Promoter	Struhl and Basler, 1993	N.A.
pBPw.Act5CP-GCaMP6m	This paper	N.A.
pCFD5 vector	Addgene	Plasmid #73914
Software and algorithms
FIJI	Schindelin, et al., 2012	https://imagej.net/Fiji
Microsoft Excel	Microsoft	https://www.microsoft.com/en-us/microsoft-365/excel
GraphPad Prism 9	Graphpad	https://www.graphpad.com/scientific-software/prism/
Affinity Designer	Affinity Serif	https://affinity.serif.com/en-us/designer/
Inkscape	Inkscape	https://inkscape.org/
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html
Wolfram Mathematica	Wolfram	https://www.wolfram.com/mathematica/
CRISPR Fly Design	Port, et al., 2014	www.crisprflydesign.org
JBrowse	Skinner, et al. 2009	https://flybase.org/jbrowse/
Other
Cell Signaling Technologies Protease inhibitor cocktail	Cell Signaling Technology	Cat#5871S
MS-Safe Protease and Phosphatase inhibitor	Millipore Sigma	MSSAFE
Protease Inhibitor Cocktail Set III	Millipore Sigma	Cat#539134
Protease Inhibitor Cocktail Set VI	Millipore Sigma	539133