Table 1.

MALDI-TOF MS structural data and endogenous murine CD14-TLR4 engagement by bacterial strain. Structural data reported for each strain: 1) The primary m/z peak value, 2) the number of acyl chains predicted for the m/z peak value reported, 3) mono-phosphorylation of the lipid A di-glucosamine backbone, and 4) the addition of phosphoethanolamine (PEtn) to the lipid A di-glucosamine backbone. CD14-TLR4 engagement is reported as Yes/No compared to E. coli.

Strain Identification	1° ionizable m/z peak value	Predicted # acyl chains	Minor mono-${\mathrm{PO}}_4^{3-}$ (Yes/No)	PEtn addition (Yes/No)	Cardiolipin (Yes/No)	CD14 internalization	TLR4/MD2 dimerization
E. coli	1798	6	No	No	No	Yes	Yes
Colwellia 1	2048	7	Yes	No	No	No	No
Shewenella 1	1727	6	Yes	No	No	No	No
Moritella 27	1792	6	Yes	No	No	No	No
Moritella 9	1755	6	Yes	Yes	No	No	No
Moritella 28	1739	6	No	No	No	No	No
Colwellia 3	2047	7	Yes	Yes	No	No	No
Moritella 23	1739	6	No	No	No	No	No
Moritella 11	1798	6	Yes	Yes	No	No	No
Moritella 32	1768	6	No	No	No	No	No
Colwellia 2	2020	7	Yes	No	No	No	No
Moritella 36	1739	6	No	No	No	No	No
Moritella 8	1658	6	No	No	No	No	Yes
Moritella 4	1766	6	Yes	Yes	No	No	Yes
Moritella 31	1740	6	Yes	No	No	No	Yes
Moritella 41	1740	6	No	No	No	No	Yes
Moritella 34	1739	6	Yes	No	No	No	No
Moritella 40	1739	6	Yes	No	No	No	No
Moritella 2	1740	6	Yes	No	No	No	No
Halomonas 1	1739	6	Yes	No	No	No	No
Moritella 33	1739	6	Yes	No	No	No	No
Moritella 6	1740	6	Yes	No	No	No	No
Moritella 35	1766	6	Yes	No	No	No	Yes
Moritella 14	1790	6	Yes	No	No	No	No
Moritella 29	1740	6	Yes	No	Yes	No	No
Moritella 42	1739	6	No	No	No	No	Yes
Moritella 5	1739	6	No	No	No	No	No
Moritella 43	1769	6	Yes	No	No	No	Yes
Moritella 30	1764	6	No	No	No	No	No
Moritella 26	1766	6	Yes	No	No	No	No
Moritella 18	1767	6	Yes	No	No	No	Yes
Moritella 19	1766	6	Yes	No	No	No	Yes
Moritella 22	1739	6	Yes	No	No	No	Yes
Moritella 16	1764	6	Yes	No	No	No	No
Moritella 39	1766	6	No	No	No	No	No
Moritella 37	1766	6	No	No	No	No	Yes
Moritella 20	1767	6	Yes	No	No	No	No
Moritella 17	1790	6	No	No	Yes	Yes	Yes
Moritella 38	1768	6	Yes	No	No	Yes	Yes
Moritella 21	1659	6	No	No	Yes	Yes	Yes
Vibrio 1	1754	6	No	No	Yes	No	Yes
Moritella 45	1807	6	No	No	No	Yes	Yes
Moritella 10	1767	6	Yes	No	No	No	Yes
Moritella 44	1807	6	No	No	Yes	Yes	Yes
Moritella 7	1766	6	Yes	No	No	Yes	Yes
Moritella 25	1655	6	No	No	Yes	Yes	Yes
Moritella 3	1766	6	Yes	No	No	Yes	Yes
Moritella 15	1766	6	Yes	No	No	No	Yes
Moritella 13	1764	6	Yes	No	No	No	Yes
Moritella 12	1791	6	No	No	No	Yes	Yes
Moritella 24	1784	6	No	No	No	Yes	Yes