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The Primer Sequences Used for qRT-PCR
| Gene | Primer Sequence (5ʹ- 3ʹ) |
|---|---|
| CHL1-AS1 | Forward:5ʹ-GCCTCAGCCTCCCAAGTAGCA-3ʹ |
| Reverse:5ʹ-TAGCCAGCCGTCAGACCATCA-3ʹ | |
| hsa-miR-610 | Forward:5ʹ-ACTCCAGCTGAGCTAAATGTG-3ʹ |
| Reverse:5ʹ-CAGTGCGTGTCGTGGAGAGGT-3ʹ | |
| MDM2 | Forward:5ʹ-GGTCTGGCAGGTAGTAAGCAC-3ʹ |
| Reverse:5ʹ-AAACTTCAAGGTGGAGTAGGG-3ʹ | |
| U6 | Forward:5ʹ-CTCGCTTCGGCAGCACA-3ʹ |
| Reverse:5ʹ-AACGCTTC ACGA ATTTGCGT-3ʹ | |
| GAPDH | Forward:5ʹ-GACTCATGACCACAGTCCATGC-3ʹ |
| Reverse:5ʹ-AGAGGCAGGGATGATGTTCTG-3ʹ |
