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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2350:43–68. doi: 10.1007/978-1-0716-1593-5_4

Fig. 3.

Fig. 3

Western blot showing the inducible expression of RhoA biosensor in MDA-MB231 tet-OFF cell line, detected using anti-RhoA GTPase antibody. Lane 1: Cell lysate from cell culture maintained with 2 μg/mL Dox; Lane 2: Cell lysate from biosensor induced cells that underwent single cycle of trypsinization; Lane 3: Cell lysate from biosensor induced cells that underwent two cycles of trypsinization. Endogenous RhoA expression levels are similar in all cases