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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2350:43–68. doi: 10.1007/978-1-0716-1593-5_4

Fig. 5.

Fig. 5

Grid-based alignment of side port channel (CFP) to the bottom port views. Comparison of the original unmodified bottom port view (Top left, Camera 3 in Fig. 1) shows significant deviation from the reference image obtained from the CFP channel (Top right) at the side camera (Camera 2 in Fig. 1). Coordinate transformation based on manual determination of control points within the grid image brings the bottom port view partially into register (Bottom left). Additional X-Y linear translation shift (−51 pixels in X and + 3 pixels in Y) (Bottom right) was required to fully register the bottom port view against the reference CFP channel image (Top right). Red bar = 20 μm