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Bandpass filter specifications used for multiplex imaging of NIR FRET and CFP-YFP FRET biosensors in single living cells. “ET” designation indicates the filters were from Chroma Technology, Inc. “FF” designation indicates the filters were from Semrock, Inc.
| Channel ID | Excitation | Emission |
|---|---|---|
|
| ||
| CFP (donor) | ET436/20× | ET480/40M |
| FRET (CFP-YFP) | ET436/20× | ET535/30M |
| miRFP670 (donor) | FF01–628/32 | FF02–684/24 |
| FRET (670–720) | FF01–628/32 | FF01–794/160 |
