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Rhodamine B diffusion into MCF7 cells acquired at 25 frames per second (fps) for one minute. (a) Multiphoton intensity, (b) fluorescence lifetime, and the overlay of the two (c) are shown for times of 1 s through 16 s and 60 s of imaging, displayed with no frame averaging. To demonstrate the changes observable with 25 fps imaging, the overlay images taken between 2 and 3 s are shown in (d). Fluorescence lifetime is given in ns according to the colorbar. Scale bar represents 20 µm.
