Fig. 6.

Volumetric Growth Rate Heterogeneity. Heterogeneity analysis of pre-treatment normalized organoid volume at 48 hours for Patient 1 (P1) PDCOs. (A) Single-component Gaussian distributions were separately fit to the pre-treatment normalized PCO volumes for each of the P1 treatment groups at 48 hours. (B) Dot plots of the pre-treatment normalized organoid volume at 48 hours for PCOs in each of the P1 treatment groups. PCOs were stratified into hollow and solid morphology sub-types based on visual inspection of the images. Hollow organoids for each group are plotted using open circle markers and solid organoids are plotted using solid square markers. CN: cyanide, Cis.: cisplatin, Pac.: paclitaxel. (C) The Z-score $[(X-{\mu }_{Treatment})/{\sigma }_{Treatment}]$for all pre-treatment normalized P1 PCOs at 48 hours were calculated and stratified into hollow or solid morphological sub-types. The Z-score enables comparison between treatment groups. Statistical significance was assessed using the non-parametric Mann-Whitney U test (** for p < 0.01). Distributions of the Z-scores were plotted from the dot plot data (gramm Package, MATLAB). Table 1 shows the number of PCOs for Patient 1.