Fig. 1.

Temperature dependence of fluorescence intensity, lifetime, steady-state anisotropy and rotational correlation time for free NADH. a) The fluorescence intensity, b) long ( ${\tau }_2$ ), short ( ${\tau }_1$ ), and mean ( ${\tau }_m$ ) fluorescence lifetimes, (c) steady-state anisotropy, and (d) rotational correlation time are shown as a function of temperature. Data points in (a) and (b) show the mean of three measurements for Dataset 1 and one measurement for Dataset 2. Only one measurement of steady-state anisotropy was performed in Datasets 1 and 2 and so no error bars are shown in panel (c). Polarisation-resolved data was not obtained for Dataset 1 and so panel (d) only shows the results from Dataset 2. Where shown, error bars indicate the average over all temperatures of the standard deviation at each temperature for a specific dataset. The average error bar is shown on only one data point per Dataset to avoid the figure becoming excessively crowded.