Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Escherichia coli)	See Supplementary file 1A	This paper		See Supplementary file 1A
Strain, strain background (Caulobacter crescentus)	See Supplementary file 1A	This paper		See Supplementary file 1A
Recombinant DNA reagent	See Supplementary file 1B	This paper		See Supplementary file 1B
Sequence-based reagent	See Supplementary file 1B	This paper		See Supplementary file 1B
Antibody	Anti-GFP antibody (HRP) (Rabbit polyclonal)	Abcam	Cat# ab190584	Western blot (1:5000)
Antibody	Anti-GFP Sepharose beads	Abcam	Cat# ab69314	For ChIP-seq experiments
Antibody	Anti-FLAG antibody (HRP) (Mouse monoclonal)	Merck	Cat# A8592	Western blot (1:5000)
Antibody	Anti-FLAG M2 affinity agarose beads	Merck	Cat# A2220	For ChIP-seq experiments
Commercial assay or kit	Amersham Protran supported western blotting membranes, nitrocellulose	GE Healthcare	Cat# GE10600016	Pore size 0.45 μm, for DRaCALA assay
Commercial assay or kit	EnzChek Phosphate Assay Kit	ThermoFisher	Cat# E6646
Commercial assay or kit	Gibson Assembly Master Mix	NEB	Cat# E2611S
Commercial assay or kit	Gateway BP Clonase II enzyme mix	ThermoFisher	Cat# 11789020
Commercial assay or kit	Dip-and-Read Streptavidin biosensors SAX2	Sartorius UK	Cat# 18-5019
Commercial assay or kit	HisTrap High Performance column	GE Healthcare	Cat# GE17524801
Commercial assay or kit	HisTrap Heparin High Performance column	GE Healthcare	Cat# GE17040601
Commercial assay or kit	HiLoad 16/600 Superdex 200 pg column	GE Healthcare	Cat# GE28989335
Commercial assay or kit	0.5 mL Zeba spin desalting columns	ThermoFisher	Cat# 89,882	7K Da molecular weight cutoff
Peptide, recombinant protein	BamHI-HF	NEB	Cat# R3136S	20,000 units/mL
Peptide, recombinant protein	HindIII-HF	NEB	Cat# R3104S	20,000 units/mL
Chemical compound, drug	Benzonase nuclease	Merck	Cat# E1014	250 units/µL
Chemical compound, drug	CTP	ThermoFisher	Cat# R0451	100 mM solution
Chemical compound, drug	CTPγS	Jena Bioscience		Custom synthesis (purity ≥96%)
Chemical compound, drug	P32-α-CTP	Perkin Elmer	Cat# BLU008H250UC	3,000 Ci/mmol, 10 mCi/mL, 250 µCi
Chemical compound, drug	Bismaleimidoethane (BMOE)	ThermoFisher	Cat# 22323	Dissolved in DMSO
Chemical compound, drug	AcTEV protease	ThermoFisher	Cat# 12575015	10 units/µL
Software, algorithm	BLItz Pro	Molecular Devices	Cat# 50-0156	Version 1.2
Software, algorithm	AIMLESS	Evans and Murshudov, 2013	http://www.ccp4.ac.uk/	Version 0.7.4
Software, algorithm	BUCCANEER	Cowtan, 2006	http://www.ccp4.ac.uk/	Version 1.6.10
Software, algorithm	Coot	Emsley and Cowtan, 2004	http://www.ccp4.ac.uk/	Version 0.9.5
Software, algorithm	CHAINSAW	Stein, 2008	http://www.ccp4.ac.uk/	Version 7.0.077
Software, algorithm	DIALS	Winter et al., 2018	https://dials.github.io	Version 3.1.0
Software, algorithm	Excel 2016	Microsoft	RRID:SCR_016137	Version 16.0
Software, algorithm	GraphPad Prism 8	GraphPad Software	RRID:SCR_002798	Version 8
Software, algorithm	ImageJ	NIH	https://imagej.net/ RRID:SCR_003070	Version 1.50
Software, algorithm	Image Studio Lite	LI-COR Biosciences	RRID:SCR_013715	Version 5.2
Software, algorithm	PISA	Krissinel, 2015	http://www.ccp4.ac.uk/pisa/	Version 2.1.1
Software, algorithm	MolProbity	Williams et al., 2018	http://molprobity.biochem.duke.edu/	Version 4.5
Software, algorithm	PHASER	McCoy et al., 2007	https://www.phenix-online.org/	Version 2.8.2
Software, algorithm	PyMOL	The PyMOL Molecular Graphics System	https://pymol.org/2/	Version 2.4.0
Software, algorithm	R	R Foundation for Statistical Computing	https://www.r-project.org/	Version 3.2.4
Software, algorithm	REFMAC5	Murshudov et al., 1997	http://www.ccp4.ac.uk/	Version 5.8.0258
Software, algorithm	SCULPTOR	Bunkóczi and Read, 2011	http://www.ccp4.ac.uk/	Version 0.0.3
Software, algorithm	XDS	Kabsch, 2010	https://xds.mr.mpg.de/	Version Nov11-2017
Software, algorithm	XIA2	Winter, 2009	https://xia2.github.io/index.html	Version 0.3.7.0