Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | WTC | Gladstone Institutes | Human iPSC line | |
| Antibody | Primary Anti-MYBPC3 Mouse (monoclonal) |
Santa Cruz | Sc-137237 | 1:200 |
| Antibody | Primary Anti-ACTN2 Rabbit (monoclonal) |
Thermo Fisher Scientific | 701914 | 1:200 |
| Antibody | Secondary Donkey anti-Rabbit IgG (H+L) Alexa Fluor 594 |
Thermo Fisher Scientific | A-21202 | 1:500 |
| Antibody | Secondary Donkey anti-Mouse IgG (H+L) Alexa Fluor 488 |
Thermo Fisher Scientific | A-21207 | 1:500 |
| Sequence-based reagent | MYH7 | Thermo Fisher Scientific | Hs01110632_m1 | TaqMan qPCR probe, cardiac marker |
| Sequence-based reagent | MYH6 | Thermo Fisher Scientific | Hs01101425_m1 | TaqMan qPCR probe, cardiac marker |
| Sequence-based reagent | TNNI3 | Thermo Fisher Scientific | Hs00165957_m1 | TaqMan qPCR probe, cardiac marker |
| Sequence-based reagent | TNNI1 | Thermo Fisher Scientific | Hs00913333_m1 | TaqMan qPCR probe, cardiac marker |
| Sequence-based reagent | MYBPC3 | Thermo Fisher Scientific | Hs00165232_m1 | TaqMan qPCR probe, cardiac marker |
| Sequence-based reagent | TNNT2 | Thermo Fisher Scientific | Hs00943911_m1 | TaqMan qPCR probe, cardiac marker |
| Sequence-based reagent | GAPDH | Thermo Fisher Scientific | Hs99999905_m1 | TaqMan qPCR probe, housekeeping marker |
| Chemical compound, drug | CHIR-99021 | Selleckchem | S1263 | Small molecule |
| Chemical compound, drug | Y-27632 | Selleckchem | S1049 | Small molecule |
| Chemical compound, drug | IWP2 | Sigma | I0536 | Small molecule |
| Chemical compound, drug | Blasticidine S hydrochloride | Thermo Fisher Scientific | 15205 | Small molecule |
| Chemical compound, drug | Bortezomib | Selleckchem | S1013 | Small molecule |
| Chemical compound, drug | Doxorubicin | Selleckchem | S1208 | Small molecule |
| Chemical compound, drug | Givinostat | Selleckchem | S2170 | Small molecule |
| Chemical compound, drug | Bafilomycin | Selleckchem | S1413 | Small molecule |
| Chemical compound, drug | Paclitaxol | Selleckchem | S1150 | Small molecule |
| Chemical compound, drug | Cisapride | Selleckchem | S4751 | Small molecule |
| Chemical compound, drug | Sorafenib | Selleckchem | S7397 | Small molecule |
| Chemical compound, drug | FDA-approved Drug Library | Selleckchem | L1300 | Compound library |
| Chemical compound, drug | Discovery Diversity Set | Enamine | DDS-10-Y-10 | Compound library |
| Chemical compound, drug | Dimethyl sulfoxide (DMSO) | Sigma | D8418 | Solvent |