Fig. 5. Myogenic gene expression: Pax3, Pax7, MYOD, MYOG, MYF4, and MRF5 gene expression in BSC cultured with ciglitazone in DMEM containing 2% HS after 96 h of incubation.

CON, DMEM + 2% HS; CL, CON + 5 µM ciglitazone; CM, CON + 10 µM ciglitazone; CH, CON + 20 µM ciglitazone. The results of gene expression were consistent with protein expression. The bars are the means ± SE. ^a–dIndicate a significant difference (p < 0.01) within an item compared with undifferentiated cells. The results are representative of six independent experiments. Pax3, Paired box 3; Pax7, Paired box 7; MYOD, myogenic differentiation-1; MYOG, myogenin; MYF4, myogenin-4; MRF5, myogenic regulatory factor-5; BSC, bovine satellite cells; DMEM, Dulbecco’s modified Eagle’s medium; HS, horse serum.