Skip to main content
. 2021 Jul 31;63(4):766–777. doi: 10.5187/jast.2021.e64

Fig. 6. Bez decreased mitochondrial biogenesis in aged blastocysts.

Fig. 6.

(A) DNA was stained with Hoechst 33342 (blue), mitochondria were stained with mitotrackerRed (red), and TOM20 was stained with TOM20 antibody (green). (B) No difference was observed between control (n = 28) and treatment (n = 28) on mitochondria, TOM20, and ratio of mitochondria/TOM20. (C) mtDNA copy number was measured via RT-PCR. No difference was observed on mtDNA copy number during treatment (n = 33) in oocyte (n = 48). (D) Mitochondria was decreased, TOM20 was increased, and ratio of mitochondria/TOM20 was decreased in the presence of Bez (n = 22) in aged blastocysts (n = 21). (E) mtDNA copy number was decreased in the presence of Bez (n = 54) in blastocysts (n = 86). All experiments were replicated at thrice times and data are presented as means ± SEM. *p < 0.05, **p < 0.01. MII, metaphase II; Bez, bezafibrate; RT-PCR, real-time polymerase chain reaction.