Table 2.
Kinetic parameters of IlAXE1.
| Substrate | Kinetic parameters | ||||
|---|---|---|---|---|---|
|
Km (mM) |
Vmax (mM・min -1) |
kcat (s -1) |
kcat/ Km (mM -1・s -1) |
||
| 4-Metylumbelliferyl acetate | MUAc | 0.4 ± 0.1 | 0.057 ± 0.010 | 33.8 ± 5.7 | 85.5 |
| p-Nitrophenyl acetate | pNPAc | 1.4 ± 0.3 | 0.049 ± 0.007 | 29.1 ± 4.3 | 20.6 |
| α-Naphtyl acetate | αNAc | N.D. | N.D. | N.D. | 12.1 ± 8.1 |
Km and k cat values are shown with standard deviations from three independent experiments. N.D.: values could not be obtained due to the low solubility of αNA at the concentration above 1.0 mM.