Fig. 2. Optimal pH and temperature of BlAga3.

　A, pH dependence of BlAga3 activity in various buffers at 40 °C for 20 min. Sodium acetate buffer (closed circle and solid line), sodium phosphate buffer (closed square and dashed line), and Tris-HCl buffer (closed triangle and dotted line) were used. Enzyme activities are expressed as a percentage of the activity in sodium acetate buffer at pH 6.0. B, the temperature dependence of BlAga3 activity at pH 6.0 for 20 min. The enzymatic activities are expressed as the percentage of the activity at 50 °C.