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Example of dilutions used for the preparation of the calibration curve
| AMC final concentration (μM) | Dilution factor | Volume of 100 μM AMC solution per well (μl) | Volume of assay solution per well (μl) | Total volume (μl) |
|---|---|---|---|---|
| 25 | 4 | 50.0 | 150.0 | 200 |
| 12.5 | 8 | 25.0 | 175.0 | 200 |
| 6.25 | 16 | 12.5 | 187.5 | 200 |
| 3.125 | 32 | 6.2 | 193.7 | 200 |
| 1.5625 | 64 | 3.1 | 196.9 | 200 |
| 0 | - | 0 | 200.0 | 200 |
