Table 3.
Example of raw data obtained from the testing of the inhibitory action of ebselen
| Well (ebselen, μM) |
A1 (20) |
A2 (10) |
A3 (5) |
A4 (2.5) |
A5 (1.25) |
A6 (0.63) |
A7 (0.31) |
A8 (0.16) |
A9 (0.08) |
A10 (0.04) |
A11 (0.02) |
A12 (0, control) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time (min) | Fluorescence (RFU) | |||||||||||
| 0.0 | 11 | 10 | 13 | 25 | 189 | 282 | 299 | 308 | 197 | 211 | 211 | 221 |
| 0.5 | 11 | 9 | 13 | 28 | 219 | 331 | 350 | 371 | 246 | 267 | 266 | 285 |
| 1.0 | 11 | 10 | 13 | 30 | 248 | 379 | 398 | 419 | 301 | 319 | 325 | 344 |
| 1.5 | 11 | 10 | 13 | 34 | 273 | 432 | 453 | 481 | 355 | 372 | 379 | 408 |
| 2.0 | 11 | 10 | 12 | 36 | 303 | 482 | 505 | 531 | 414 | 432 | 444 | 477 |
| 2.5 | 10 | 10 | 13 | 38 | 337 | 536 | 561 | 582 | 474 | 491 | 507 | 537 |
| 3.0 | 11 | 10 | 12 | 41 | 365 | 584 | 614 | 641 | 528 | 551 | 567 | 600 |
| 3.5 | 11 | 10 | 12 | 45 | 395 | 639 | 670 | 705 | 587 | 609 | 635 | 679 |
| 4.0 | 11 | 10 | 12 | 48 | 431 | 700 | 734 | 763 | 647 | 678 | 704 | 756 |
| 4.5 | 11 | 10 | 12 | 50 | 461 | 758 | 790 | 819 | 724 | 751 | 768 | 822 |
| 5.0 | 11 | 10 | 11 | 54 | 496 | 814 | 846 | 871 | 770 | 808 | 827 | 891 |
| 5.5 | 11 | 9 | 11 | 59 | 530 | 881 | 906 | 937 | 833 | 865 | 900 | 961 |
| 6.0 | 12 | 10 | 11 | 63 | 558 | 922 | 961 | 989 | 886 | 930 | 964 | 1021 |
| 6.5 | 11 | 9 | 11 | 66 | 585 | 982 | 1024 | 1042 | 945 | 1001 | 1025 | 1090 |
| 7.0 | 12 | 10 | 11 | 72 | 622 | 1040 | 1086 | 1113 | 1016 | 1089 | 1100 | 1171 |
| 7.5 | 12 | 10 | 10 | 77 | 647 | 1111 | 1153 | 1183 | 1089 | 1156 | 1162 | 1242 |
| 8.0 | 11 | 10 | 11 | 80 | 683 | 1159 | 1209 | 1247 | 1140 | 1224 | 1239 | 1319 |
| 8.5 | 11 | 10 | 11 | 85 | 720 | 1216 | 1262 | 1293 | 1198 | 1303 | 1297 | 1370 |
| 9.0 | 11 | 9 | 10 | 87 | 748 | 1280 | 1329 | 1350 | 1257 | 1356 | 1371 | 1453 |
| 9.5 | 11 | 9 | 10 | 93 | 786 | 1346 | 1398 | 1428 | 1323 | 1432 | 1446 | 1515 |
| 10.0 | 11 | 10 | 11 | 96 | 814 | 1408 | 1479 | 1484 | 1387 | 1503 | 1512 | 1585 |
| Calculated slope (RFU/min) | 0.02 | −0.02 | −0.28 | 7.23 | 63 | 112 | 116 | 117 | 120 | 130 | 130 | 137 |
| R2 | 0.201 | −0.24 | −0.90 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 | 0.99 |