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Example of fluorescence data obtained for the preparation of the standard curve correlating AMC concentrations and fluorescence values
| AMC concentration (μM) | Replicate | Fluorescence (RFU) | Mean fluorescence (RFU) |
|---|---|---|---|
| 25 | A | 13917 | 13903 |
| B | 13889 | ||
| 12.5 | A | 7070 | 7172 |
| B | 7273 | ||
| 6.25 | A | 3620 | 3648 |
| B | 3675 | ||
| 3.125 | A | 1798 | 1825 |
| B | 1852 | ||
| 1.5625 | A | 9123 | 911 |
| B | 909 | ||
| 0 | A | 11 | 8.8 |
| B | 5 |
