Fig. 3.

Klotho enhance the proliferation ability and secretory levels of BMSCs. (a) The cell proliferation ability of BMSCs in three groups was determined by CCK-8 assay at 24 h, 48 h and 96 h (n = 9). (b) Cell cycle analysis of BMSCs, by comparing the percentage of cells, in each group, in S phase and G2/M phase and G0/G1 phase (n = 3). (c) Representative immunoblot for Cyclin D1 in each group. (d) The secretory levels of insulin-like growth factor-1 (IGF-1) Hepatocyte growth factor (HGF), vascular endothelial growth factor-A (VEGF-A) and by BMSCs in each group (n = 3). Data presented as mean ± standard deviation. ∗P < 0.05 versus BMSCs, BMSCs-EV.