Extended Data Fig. 8. Comparison of the two types of TS on the production of taxadiene in E. coli.

a, Taxadiene-producing E. coli T2 (harboring pMH1, pFZ81, and pXC02) was constructed by coexpressing nine genes (AtoB, ERG13, tHMG1, ERG12, ERG8, MVD1, IdI, GGPPS, and TbTS) in E. coli²⁹, while E. coli TS2 was generated by replacing TbTS with TS2 in E. coli T2; b, The cell concentrations of the strains E. coli T2 and TS2 were measured by OD₆₀₀ at set intervals (at 8, 13, 22, 37, 46, 60, 72 and 84 hours); c, The titers of taxadiene produced by E. coli T2 and TS2 in shaking flasks. TbTS, a T. brevifolia taxadiene synthase that shares 98.42 % amino acid sequence identity with TS1, represents type I TSs, while TS2, sharing 77 % protein sequence identity with TS1, represents type II TSs. Error bars show standard error (n = 4 independent biological replicates).