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. 2021 Aug 16;11:16574. doi: 10.1038/s41598-021-96102-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of stress responsive genes in wild-type and BoCRP1 transgenic lines. (a–h) depicts the transcript levels of SOD, POD, CAT, ProT1, DREB1, LTP1, ERD15-2 and LEA in wild-type and BoCRP1 overexpression lines. Six week old seedlings of transgenic lines and wild-type plants treated with cold (4 °C) or 25 °C (control) for 3 days were used to collect the samples for RNA extraction. For relative expression levels, 2^−∆∆CT method was employed using β-Tubulin as internal control to normalize the expression levels of target genes. Data represents the mean ± SD of three biological replicates. Each sample replicate composite of leaves was collected from a total of 12 seedlings. WT represents the wild-type tomato, OE1, OE2 and OE3 represent three independent putative BoCRP1 transgenic tomato lines. Asterisks represents the significant differences between wild-type and transgenic lines. *P < 0.05; **P < 0.01, ***P < 0.001 Tukey's Multiple Comparison Test.