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. 2021 Aug 16;11:16525. doi: 10.1038/s41598-021-96152-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Vitamin D deficiency aggravates the induction of pro-fibrotic factors by BLM. (A) Real-time RT-PCR quantitation of mRNA expressions of pro-fibrotic genes. *P < 0.05, **P < 0.01, ***P < 0.001, versus VDS group; ^&&P < 0.05, ^&&&P < 0.001, versus VDD group, ^#P < 0.05, ^##P < 0.01, versus VDS + BLM group; by two-way ANOVA. n = 3–4 each group. (B,C) Western blot analysis (B) and densitometric quantitation (C) of α-SMA, TGF-β1, Col I and FN in the lung lysates. *P < 0.05, **P < 0.01, ***P < 0.001, versus VDS group; ^&&P < 0.05, ^&&&P < 0.001, versus VDD group; ^##P < 0.01, ^###P < 0.001, versus VDS + BLM group; by two-way ANOVA and Kruskal–Wallis one-way ANOVA (for α-SMA levels). n = 5–6 each group.